Feasibility of a Multi-Laboratory Model of Middle Cerebral Artery Thromboembolic Stroke with Thrombolysis: TE-MCAo

多实验室开展大脑中动脉血栓栓塞性卒中溶栓模型（TE - MCAo）的可行性研究

No single animal stroke model satisfies all needs of translational stroke investigation. While the nylon filament model is widely accepted in preclinical translational stroke research, thromboembolic models have more physiological relevance. Thromboembolic models are technically difficult, time consuming, and show variable results, making them problematic for multi-laboratory preclinical network use. We sought to develop and validate a multi-laboratory thromboembolic middle cerebral artery occlusion model that encompasses vessel occlusion and subsequent thrombolysis. We established the feasibility and reproducibility of a multi-laboratory rodent thromboembolic model but further development is needed to improve the success rate and lower the mortality rate before this model can be accepted widely.

没有单一的动物卒中模型能满足转化性卒中研究的所有需求。虽然尼龙线栓模型在临床前转化性卒中研究中被广泛接受，但血栓栓塞模型具有更高的生理相关性。血栓栓塞模型技术上难度大、耗时长，且结果不稳定，使其难以用于多实验室临床前研究网络。我们试图开发并验证一种涵盖血管阻塞及后续溶栓过程的多实验室血栓栓塞性大脑中动脉闭塞模型。我们证实了多实验室啮齿动物血栓栓塞模型的可行性和可重复性，但在该模型被广泛接受之前，还需要进一步改进以提高成功率并降低死亡率。

REF: Lin M, Bhuiyan M, McCurry C, et al. Feasibility of a Multi-Laboratory Model of Middle Cerebral Artery Thromboembolic Stroke with Thrombolysis: TE-MCAo. Transl Stroke Res. 2026;17(1):20. Published 2026 Jan 30. doi:10.1007/s12975-025-01407-4 PMID: 41611917 PMCID: PMC12855246

Predictive Utility of Cerebral Blood Flow Transients in Experimental Stroke

实验性卒中中脑血流瞬变的预测效用

A gap in developing novel preclinical treatment strategies for ischemic stroke is predicting long-term outcome in experimental stroke models early during ischemia to reduce heterogeneity and sample size. Besides saving costs through improved risk stratification, reducing the number of animals is a requirement of the 3Rs principle. In this secondary analysis, we analyzed 28 Sprague-Dawley rats of a prospective data base that underwent 90-minute filament-occlusion of the middle cerebral artery (MCAO) to assess the predictive power of early variables at 30, 60, and 90 min after occlusion. In summary, we have found that early risk stratification during MCAO in rats is possible using minimally invasive biomarkers. This would enable, in particular, the early sorting out of animals with a low risk of cortical infarction in neuroprotection studies, where these animals typically distort the statistical results.

开发缺血性中风新型临床前治疗策略的一个空白点在于，在缺血早期对实验性中风模型的长期结局进行预测，以降低异质性和样本量。除了通过改善风险分层节省成本外，减少实验动物数量也是“3R原则”的要求。在这项二次分析中，我们分析了一个前瞻性数据库中的28只Sprague - Dawley大鼠，这些大鼠接受了90分钟的大脑中动脉细丝阻塞术（MCAO），以评估阻塞后30分钟、60分钟和90分钟时早期变量的预测能力。总之，我们发现使用微创生物标志物对大鼠MCAO期间进行早期风险分层是可行的。这尤其能够在神经保护研究中早期筛选出皮质梗死风险较低的动物，因为这些动物通常会干扰统计结果。

REF: Lückl J, Szücs M, Rarosi F, Salehzadeh A, Dreier JP. Predictive Utility of Cerebral Blood Flow Transients in Experimental Stroke. Transl Stroke Res. 2026;17(1):19. Published 2026 Jan 22. doi:10.1007/s12975-025-01410-9 PMID: 41566111 PMCID: PMC12823744

Outcomes of Bypass Surgery in Patients with Moyamoya Syndrome Secondary To Sickle Cell Disease: a Multicenter Study

镰状细胞病继发烟雾综合征患者搭桥手术的结局：一项多中心研究

Moyamoya syndrome (MMS) associated with sickle cell disease (SCD) is a severe vasculopathy that significantly increases stroke risk. While cerebral revascularization is increasingly considered in this population, concerns about perioperative safety and long-term outcomes have limited its use in clinical practice. Surgical revascularization for MMS in patients with SCD does not confer additional procedural risk and yields outcomes comparable to those of patients without SCD. These findings support the role of bypass surgery as a viable treatment option in this high-risk population.

与镰状细胞病（SCD）相关的烟雾综合征（MMS）是一种严重的血管病变，会显著增加中风风险。虽然在这一人群中越来越多地考虑进行脑血运重建，但对围手术期安全性和长期预后的担忧限制了其在临床实践中的应用。对SCD患者的MMS进行手术血运重建不会带来额外的手术风险，且预后与非SCD患者相当。这些发现支持旁路手术作为这一高风险人群可行治疗选择的作用。

REF: Musmar B, Roy JM, Abdalrazeq H, et al. Outcomes of Bypass Surgery in Patients with Moyamoya Syndrome Secondary To Sickle Cell Disease: a Multicenter Study. Transl Stroke Res. 2026;17(1):18. Published 2026 Jan 21. doi:10.1007/s12975-026-01412-1 PMID: 41563633

Recent Advances in Mitochondrial Transplantation for Ischemic Stroke: A Review of in Vitro and in Vivo Studies

线粒体移植治疗缺血性脑卒中的最新进展：体外和体内研究综述

Ischemic stroke is a common and devastating disease that imposes a huge burden on global health and the economy. Mitochondrial dysfunction is a key feature of cerebral ischemia-reperfusion injury (CIRI). Mitochondrial transplantation, an emerging neuroprotective strategy that introduces exogenous mitochondria into a living organism, has shown great potential in various neurological diseases. However, significant challenges persist, including the lack of standardized dosing regimens and unclear mechanisms regarding long-term mitochondrial engraftment. While initial clinical trials have demonstrated the safety of this approach, the field is currently at a pivotal juncture requiring rigorous translation from preclinical success to proven clinical efficacy. This review summarizes the in vitro and in vivo research findings on mitochondrial transplantation in stroke models, with the aim of providing a basis for the clinical translation of this technology.

缺血性中风是一种常见且具有毁灭性的疾病，给全球健康和经济带来了巨大负担。线粒体功能障碍是脑缺血再灌注损伤（CIRI）的一个关键特征。线粒体移植是一种新兴的神经保护策略，它将外源性线粒体引入生物体，已在多种神经系统疾病中显示出巨大潜力。然而，仍然存在重大挑战，包括缺乏标准化的给药方案以及长期线粒体植入机制不明。虽然初步临床试验已证明这种方法的安全性，但该领域目前正处于一个关键转折点，需要将临床前的成功严格转化为已证实的临床疗效。本综述总结了线粒体移植在中风模型中的体外和体内研究成果，旨在为该技术的临床转化提供依据。

REF: Mei Z, Zhong W, Deng T, et al. Recent Advances in Mitochondrial Transplantation for Ischemic Stroke: A Review of in Vitro and in Vivo Studies. Transl Stroke Res. 2026;17(1):17. Published 2026 Jan 21. doi:10.1007/s12975-025-01401-w PMID: 41563620

Collagen Imaging in Human Intracranial Aneurysms – a Translational Proof-of-Concept Study

人颅内动脉瘤的胶原蛋白成像——一项转化性概念验证研究

Unruptured intracranial aneurysms (IAs) are highly prevalent in the general population and there is an unmet need for radiological surrogates for the assessment of an increased risk of rupture. High turnover of type I collagen, i.e. abundant proportions of newly synthesized, immature collagen, has been linked to structural instability of the IA wall. This study aimed to test a newly synthesized positron emission tomography tracer that could selectively visualize immature type I collagen in human IAs as a marker for structural instability and thereby increased rupture risk. [68Ga]Ga-NODA-GA-PEG1-collagelin could serve as a complementary, noninvasive molecular imaging tool for personalized assessment of IA instability, but animal studies are required before in vivo use of [68Ga]Ga-NODA-GA-PEG1-collagelin in humans.

未破裂颅内动脉瘤（IAs）在普通人群中非常普遍，目前迫切需要有影像学替代指标来评估破裂风险的增加情况。I型胶原蛋白的高周转，即新合成的未成熟胶原蛋白占比高，与颅内动脉瘤壁的结构不稳定有关。本研究旨在测试一种新合成的正电子发射断层扫描示踪剂，该示踪剂可选择性地显示人类颅内动脉瘤中的未成熟I型胶原蛋白，将其作为结构不稳定的标志物，进而评估破裂风险的增加情况。[68Ga]Ga - NODA - GA - PEG1 - 胶原素可作为一种补充性的非侵入性分子成像工具，用于个性化评估颅内动脉瘤的不稳定性，但在将[68Ga]Ga - NODA - GA - PEG1 - 胶原素用于人体体内之前，需要进行动物实验。

REF: Hackenberg KAM, Willett N, Seibold U, et al. Collagen Imaging in Human Intracranial Aneurysms - a Translational Proof-of-Concept Study. Transl Stroke Res. 2026;17(1):16. Published 2026 Jan 20. doi:10.1007/s12975-025-01403-8 PMID: 41557196

Association between Lipid Accumulation Product and 3-Month Functional Outcome after Ischemic Stroke: the Obesity-Stroke Paradox

脂质蓄积产物与缺血性脑卒中后3个月功能结局的关联：肥胖 - 脑卒中悖论

This study is to explore the association of lipid accumulation product (LAP) with 3-month functional outcome and etiologic subtypes in patients with acute ischemic stroke. Patients with acute ischemic stroke admitted from July 1, 2020, to June 30, 2022 were enrolled in this study. Concurrently, patients with elevated LAP levels were more likely to experience SAO as the etiological subtype of their stroke.

本研究旨在探讨脂质蓄积产物（LAP）与急性缺血性脑卒中患者3个月功能结局及病因亚型的关联。本研究纳入了2020年7月1日至2022年6月30日收治的急性缺血性脑卒中患者。同时，LAP水平升高的患者更有可能以穿支动脉疾病（SAO）作为其脑卒中的病因亚型。

REF: Ren Y, Zhou Y, Gu TY, et al. Association between Lipid Accumulation Product and 3-Month Functional Outcome after Ischemic Stroke: the Obesity-Stroke Paradox. Transl Stroke Res. 2026;17(1):15. Published 2026 Jan 14. doi:10.1007/s12975-025-01396-4 PMID: 41530484

Internal Capsule Involvement Explains the Prognostic Impact of ASPECTS after Endovascular Thrombectomy for Large-Core Infarction

内囊受累解释了大核心梗死血管内取栓术后急性卒中早期CT评分的预后价值

The prognostic implications of Alberta Stroke Program Early CT Score (ASPECTS) subscores in patients with large-core ischemic stroke undergoing endovascular thrombectomy (EVT) remain uncertain. We hypothesized that specific ASPECTS regions substantially influence functional outcomes after EVT and therefore sought to identify which individual subscores are associated with outcome and whether any of them account for the association between total ASPECTS and functional outcomes. Internal capsule (IC) involvement independently predicts poor outcomes after EVT for large-core stroke and substantially explains the effect of total ASPECTS on prognosis.

对于接受血管内血栓切除术（EVT）的大核心梗死型缺血性卒中患者，阿尔伯塔卒中项目早期CT评分（ASPECTS）各亚评分的预后意义仍不确定。我们假设特定的ASPECTS区域会显著影响EVT后的功能结局，因此试图确定哪些单个亚评分与结局相关，以及是否有亚评分能够解释总ASPECTS评分与功能结局之间的关联。内囊（IC）受累可独立预测大核心梗死型卒中患者EVT后预后不良，并在很大程度上解释了总ASPECTS评分对预后的影响。

REF: Lee K, Choi W, Hwang YH, et al. Internal Capsule Involvement Explains the Prognostic Impact of ASPECTS after Endovascular Thrombectomy for Large-Core Infarction. Transl Stroke Res. 2026;17(1):14. Published 2026 Jan 10. doi:10.1007/s12975-025-01402-9 PMID: 41518586

Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cells Promote Functional Recovery after Experimental Intracerebral Hemorrhage Via Local and Systemic Immunomodulation

静脉注射人脐带来源间充质基质细胞通过局部和全身免疫调节促进实验性脑出血后的功能恢复

Intracerebral hemorrhage (ICH) is associated with high mortality and disability, and current treatments offer limited benefits for functional recovery. Human umbilical cord-derived mesenchymal stromal cell (hUCMSC) have strong proliferative, neuroprotective, and immunomodulatory properties, making them attractive for clinical translation. We evaluated the therapeutic effects of intravenously administered hUCMSCs in a collagenase-induced ICH model using male mice. Our results suggest that intravenous hUCMSC administration at a higher dose confers robust neuroprotection through coordinated local and systemic immunomodulation, providing translational insights for clinical MSC therapy in ICH.

脑出血（ICH）与高死亡率和高致残率相关，目前的治疗方法对功能恢复的益处有限。人脐带间充质基质细胞（hUCMSC）具有强大的增殖、神经保护和免疫调节特性，使其在临床转化方面颇具吸引力。我们在胶原酶诱导的雄性小鼠脑出血模型中评估了静脉注射hUCMSC的治疗效果。我们的研究结果表明，较高剂量静脉注射hUCMSC可通过协同的局部和全身免疫调节作用提供强大的神经保护，为脑出血的临床间充质干细胞治疗提供了转化性见解。

REF: Tsuji S, Kuramoto Y, Takeda Y, Doe N, Yamahara K, Yoshimura S. Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cells Promote Functional Recovery after Experimental Intracerebral Hemorrhage Via Local and Systemic Immunomodulation. Transl Stroke Res. 2026;17(1):13. Published 2026 Jan 9. doi:10.1007/s12975-025-01408-3 PMID: 41507510 PMCID: PMC12783308

The Hyperacute Phase of Cerebral Infarction in a Swine Model Investigated with Cone-Beam CT Perfusion Imaging

锥形束 CT 灌注成像研究猪模型脑梗死超急性期情况

In the clinical practice, patients hospitalized within 60 minutes from onset of stroke are rarely seen. This study investigated this phase in a swine middle cerebral artery (MCA) occlusion model using cone-beam CT perfusion imaging. Our results indicate that perfusion parameters in the hyperacute stroke phase can be investigated in continuity with cone-beam CT perfusion imaging in a swine stroke model. Compensatory mechanisms of CBV to maintain CBF started immediately after occlusion and MTT increased but could not counteract infarct development.

在临床实践中，很少见到发病后 60 分钟内入院的中风患者。本研究利用锥形束 CT 灌注成像技术，在猪大脑中动脉（MCA）闭塞模型中对这一阶段进行了研究。我们的研究结果表明，在猪中风模型中，可利用锥形束 CT 灌注成像技术对超急性中风阶段的灌注参数进行连续性研究。闭塞发生后，脑血容量（CBV）维持脑血流量（CBF）的代偿机制立即启动，平均通过时间（MTT）延长，但无法阻止梗死的发展。

REF: Nagayama G, Koseki H, Ikemura A, et al. The Hyperacute Phase of Cerebral Infarction in a Swine Model Investigated with Cone-Beam CT Perfusion Imaging. Transl Stroke Res. 2026;17(1):12. Published 2026 Jan 8. doi:10.1007/s12975-025-01409-2 PMID: 41505060 PMCID: PMC12783248